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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in lots of tumors, which ends up in chromosome instability, and therefore may well contribute to tumorigenesis. This protein was identified to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a vital position in endorsing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two added splice variants have been claimed but detailed nucleotide sequence information isn't nonetheless offered.
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Blocking peptides are peptides that bind especially on the target antibody and block antibody binding. These peptide commonly contains the epitope regarded because of the antibody.
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This guideline outlines further possible optimization of mobile and immunoassay parameters to be certain the very best effects are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make sure the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is precise.
Focus on Data Cyclin E belongs for the very conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates for the G1-S period boundary and it is degraded as cells progress by way of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by each antibodies...
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